Michael G. Author information Article notes Copyright and License information Disclaimer. Corresponding author. Correspondence Michael G. Katze ude. Received Mar 11; Accepted May This article has been corrected. See J Gen Virol. This article has been cited by other articles in PMC. Abstract The NS1 protein of influenza virus counters host antiviral defences primarily by antagonizing the type I interferon IFN response. Introduction Influenza A virus poses a serious global health threat.
Open in a separate window. NS1 is necessary to control production of antiviral cytokines To measure the truncation effect of the NS1 gene on cytokine production, we used a Newcastle disease virus NDV growth-inhibition bioassay. Table 1. Cell type Time p. Table 2. Discussion This is the first study to use primary human lung epithelial cells in a genomic analysis of H1N1 influenza A virus-regulated host transcriptional responses.
Methods Cell maintenance and virus preparation. HTBE and A cell culture infections. Immunoblot analysis of A cells. Expression microarray analysis. Generation of an IFN-responsive gene network in A cells.
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